Autism Ontario's 25 regional chapters form the backbone of community-based autism support across the province. Chapters operate in: Durham, Halton, Hamilton, Kingston, Kitchener-Waterloo, Lambton-Kent, London, Muskoka, Niagara, North Bay, Ottawa, Oxford, Peel, Peterborough, Simcoe, Sudbury, Thunder Bay, Toronto, Waterloo, Wellington, Windsor-Essex, York Region, and other communities.
Each chapter tailors its programming to local community needs. Urban chapters like Toronto and Ottawa offer high-frequency programming including weekly support groups, monthly social events, and regular workshops. Rural chapters like Muskoka and Peterborough focus on fewer but higher-impact events, leveraging virtual programming to extend their reach. All chapters coordinate with local OAP providers, school boards, and community agencies.